In recent years, food safety, environmental pollution, large-scale infectious diseases and other threats to human health and life safety have frequently occurred, and all sectors of society are highly concerned. In the face of these social and public problems affecting human health and life safety, it is especially important to develop rapid and sensitive detection techniques. Surface-enhanced Raman scattering (SERS) has significant technical advantages in the field of biochemical rapid detection. We have been engaged in the research of biochip technology for a long time. Uniform silver nanorods on 6×6 inches diameter substrates in different materials can be produced. Large-scale production on the substrate with a SERS enhancement factor of 109. The substrate produced is highly repeatable, the SERS intensity of the same batch of substrates changes by less than 7%, and the SERS intensity of different batches of substrates changes by less than 10%. The substrate has a simple manufacturing process, low cost, convenient storage and carrying, and the detection result is not easily interfered by external factors. Toxins, viruses, pathogens and pathogens have been successfully tested and used in food additives and medical testing. A rapid detection system for food additives based on SERS technology was established. This method can be used for rapid pretreatment and low sample quantity, and can be used for rapid and sensitive detection of food additives, pesticide residues and foodborne bacteria in vegetables and fruits.Large-scale production of a large number of substrates can greatly reduce the cost of a single test, which is conducive to the practical mass production and detection systems.